PESQUISA VETERINÁRIA BRASILEIRA

Abstract in English:

ABSTRACT.- Motta R.G., Silva A.V., Giuffrida R., Siqueira A.K., Paes A.C., Motta I.G., Listoni F.J.P. & Ribeiro M.G. 2015. [Indicators of quality and composition of informal milk marketed in the in the Southeast region of São Paulo, Brazil.] Indicadores de qualidade e composição de leite informal comercializado na região Sudeste do Estado de São Paulo. Pesquisa Veterinária Brasileira 35(5):417-423. Departamento de Higiene Veterinária e Saúde Pública, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista, Cx. Postal 560, Botucatu, SP 18618-970, Brazil. E-mail: farturavetufpr@hotmail.com The informal milk is recognized as the product marketed without inspection. We evaluated the microbiological quality, nutritional ingredients and substances inhibiting bacterial growth in 100 informal samples from cows milk, marketed in the southwest region of the state of São Paulo, Brazil. It was found that 77% of Somatic Cell Count (SCC) and 86% of Total Bacterial Count (TBC) of the samples were at odds with the maximum values required by Instruction Rules 62 of the Ministry of Agriculture and Livestock Supply. It was identified to solids contents in disagreement (36%), protein (23%), fat (38%), nonfat dry extract (43%) and 73% urea nitrogen. In 59% of the samples was detected residues of the bacterial growth inhibitors. Were isolated 240 strains of micro-organisms with a prevalence of enterobacteria, streptococci, staphylococci and fungi. The informal milk is marketed in the region without the minimum hygienic conditions and present risks to public health, and represents serious socio-economic problem for the region.

• Mastitis milk mastite leite informal

Abstract in Portuguese:

RESUMO.- Motta R.G., Silva A.V., Giuffrida R., Siqueira A.K., Paes A.C., Motta I.G., Listoni F.J.P. & Ribeiro M.G. 2015. [Indicators of quality and composition of informal milk marketed in the in the Southeast region of São Paulo, Brazil.] Indicadores de qualidade e composição de leite informal comercializado na região Sudeste do Estado de São Paulo. Pesquisa Veterinária Brasileira 35(5):417-423. Departamento de Higiene Veterinária e Saúde Pública, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista, Cx. Postal 560, Botucatu, SP 18618-970, Brazil. E-mail: farturavetufpr@hotmail.com O leite informal é reconhecido como o produto comercializado sem inspeção. Avaliou-se a qualidade microbiológica, constituintes nutricionais e presença de substâncias inibidoras do crescimento bacteriano em 100 amostras informais de leite de vacas, comercializadas na região sudoeste do estado de São Paulo, Brasil. Verificou-se que 77% da Contagem de Células Somáticas (CCS) e 86% da Contagem Bacteriana Total (CBT) das amostras estavam em desacordo com os valores máximos exigidos pela Instrução Normativa 62 do MAPA. Identificaram-se teores em desacordo para sólidos totais (36%), proteína (23%), gordura (38%), extrato seco desengordurado (43%) e nitrogênio uréico (73%). Em 59% das amostras detectou-se a presença de resíduos de inibidores do crescimento bacteriano. Isolaram-se 240 linhagens de micro-organismos com predomínio de enterobactérias, estreptococos, estafilococos e fungos. O leite informal é comercializado na região sem as mínimas condições de higiene e oferece riscos a saúde pública, além de representar sério problema sócio-econômico para região.

Abstract in English:

ABSTRACT.- Ribeiro M.G., Fernandes M.C., Paes A.C., Siqueira A.K., Pinto J.P.A.N. & Borges A.S. 2010. [Serotype characterization of Salmonella strains isolated from different affections in domestic animals.] Caracterização de sorotipos em linhagens do gênero Salmonella isoladas de diferentes afecções em animais domésticos. Pesquisa Veterinária Brasileira 30(2):155-160. Departamento de Higiene Veterinária e Saúde Pública, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista Júlio de Mesquita Filho (Unesp), Distrito de Rubião Júnior, Botucatu, SP 18618-000, Brazil. E-mail: mgribeiro@fmvz.unesp.br The serotype characterization, antimicrobial susceptibility profile, and clinical-epidemiological findings were evaluated in 53 Salmonella spp. strains isolated from 41 dogs, nine horses and three cattle presenting different clinical manifestations between 1997 at 2007. Salmonella Typhimurium (45.3%), Salmonella enterica (22.6%), Salmonella Enteritidis (7.5%), Salmonella enterica subsp. enterica 4,5,12i (5.7%), Salmonella Newport (5.7%), Salmonella Dublin (3.8%), Salmonella Agona (3.8%), Salmonella Glostrup (3.8%), Salmonella Saintpaul (1.8%) were the more common serotypes. Ciprofloxacin (100.0%), norfloxacin (100.0%) and gentamicin (100.0%) were more effective drugs while resistance of isolates was observed to ceftiofur (28.5%) and florfenicol (7.0%). The strains were isolated from animals with enteritis, urinary tract infections, septicaemia, pyometra, pneumonia and conjuntivits. The results showed the high frequency of Salmonella Typhimurium serotype in different animals studied. The study highlighted also the presence of serotypes in our animals that also have been identified in humans with salmonellosis.

• Salmonella sorotipos sensibilidade microbiana

Abstract in Portuguese:

RESUMO.- Ribeiro M.G., Fernandes M.C., Paes A.C., Siqueira A.K., Pinto J.P.A.N. & Borges A.S. 2010. [Serotype characterization of Salmonella strains isolated from different affections in domestic animals.] Caracterização de sorotipos em linhagens do gênero Salmonella isoladas de diferentes afecções em animais domésticos. Pesquisa Veterinária Brasileira 30(2):155-160. Departamento de Higiene Veterinária e Saúde Pública, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista Júlio de Mesquita Filho (Unesp), Distrito de Rubião Júnior, Botucatu, SP 18618-000, Brazil. E-mail: mgribeiro@fmvz.unesp.br Foram caracterizados os sorotipos, o perfil de sensibilidade microbiana e os achados clínico-epidemiológicos em 53 linhagens do gênero Salmonella isoladas de 41 cães, nove equinos e três bovinos, acometidos por diferentes manifestações clínicas entre 1997 e 2007. Salmonella Typhimurium (45,3%), Salmonella enterica (22,6%), Salmonella Enteritidis (7,5%), Salmonella enterica subsp enterica 4,5,12i (5,7%), Salmonella Newport (5,7%), Salmonella Dublin (3,8%), Salmonella Agona (3,8%), Salmonella Glostrup (3,8%), Salmonella Saintpaul (1,8%) foram os sorotipos encontrados. Ciprofloxacina (100,0%), norfloxacina (100,0%) e gentamicina (100,0%) foram os antimicrobianos mais efetivos, enquanto a maior resistência das linhagens foi observada para ceftiofur (28,5%) e florfenicol (7,0%). As linhagens foram isoladas de animais com enterite, infecção do trato urinário, septicemia, piometra, pneumonia e conjuntivite. Ressalta-se para o predomínio do sorovar Typhimurium nas diferentes manifestações da salmonelose nos animais. Destaca-se, também, a identificação de sorotipos nos animais que também são observados em casos de salmonelose em humanos.

Abstract in English:

ABSTRACT.- Ferreira Junior R.S., Siqueira A.K., Campagner M.V., Salerno T., Soares T.C.S., Lucheis S.B., Paes A.C. & Barraviera B. 2009. Comparison of wildlife and captivity rattlesnakes (Crotalus durissus terrificus) microbiota. [Comparação da microbiota de cascavéis (Crotalus durissus terrificus) de vida-livre e cativeiro.]Pesquisa Veterinária Brasileira 29(12):999-1003. Centro de Estudos de Venenos e Animais Peçonhentos, Universidade Estadual Paulista, Fazenda Experimental Lageado, Rua José Barbosa de Barros 1780, Botucatu, SP 18610-307, Brazil. E-mail: rseabra@cevap.org.br The study evaluated and compared the aerobic microbiota from the oral cavity, cloaca and venom of Crotalus durissus terrificus snakes, recently caught from the wild and kept under quarantine (WQ), individual captivity (IC) and collective captivity (CC). Antimicrobial drug effectiveness on isolated agents also was assayed. From group I, II and III were isolated, respectively, 29 (63.04%), 38 (90.48%) and 21 (42.86%) microorganisms from the cloaca; 15 (32.61%), 3 (7.14%) and 25 (51.02%) microorganisms from the oral cavity; and, 2 (4.35%), 1 (2.38%) and 3 (6.12%) microorganisms from venom. The most frequent bacteria were Pseudomonas aeruginosa, Proteus vulgaris and Morganella morganii, with sensitivity to amikacin, gentamicin, norfloxacin, sulfazotrin and tobramycin. Snakes kept in semi-open captivity exhibited the fewest microorganisms in oral cavities, perhaps due to the environment in captivity, with different temperature gradients, running water, absence of daily handling, circulating air, possibility of moving around, daily cleaning, and sunlight access.

• Cascavel Crotalus durissus terrificus

Abstract in Portuguese:

RESUMO.- Ferreira Junior R.S., Siqueira A.K., Campagner M.V., Salerno T., Soares T.C.S., Lucheis S.B., Paes A.C. & Barraviera B. 2009. Comparison of wildlife and captivity rattlesnakes (Crotalus durissus terrificus) microbiota. Pesquisa Veterinária Brasileira 29(12):999-1003. Centro de Estudos de Venenos e Animais Peçonhentos, Universidade Estadual Paulista, Fazenda Experimental Lageado, Rua José Barbosa de Barros 1780, Botucatu, SP 18610-307, Brazil. E-mail: rseabra@cevap.org.br Este estudo avaliou e comparou a microflora aeróbica da cavidade oral, cloaca e veneno de serpentes Crotalus durissus terrificus recém-capturadas da natureza e mantidas sob quarentena (WQ), mantidas em cativeiro coletivo (CC) e em cativeiro individual (IC). A eficácia de drogas antimicrobianas de agentes isolados foi também avaliada. Foram isolados microorganismos dos grupos I, II e III respectivamente: 29 (63.04%), 38 (90.48%) e 21 (42.86%) da cloaca; 15 (32.61%), 3 (7.14%) e 25 (51.02%) da cavidade oral, e finalmente 2 (4.35%), 1 (2.38%) e 3 (6.12%) do veneno. As bactérias mais frequentes foram Pseudomonas aeruginosa, Proteus vulgaris e Morganella morganii, com sensibilidade para amikacina, gentamicina, norfloxacina, sulfazotrina e tobramicina. Serpentes mantidas no cativeiro semi-aberto mostraram menor número de agentes infecciosos em cavidade oral, talvez devido ao ambiente de cativeiro com diferentes gradientes de temperatura, água corrente, ausência de manejo diário, ampla circulação de ar, possibilidade de movimentação pelos animais, limpeza diária e acesso ao Sol.

Abstract in English:

ABSTRACT.- Ribeiro M.G., Geraldo J.S., Langoni H., Lara G.H.B., Siqueira A.K., Salerno T. & Fernandes M.C. 2008. [Pathogenic microorganisms, somatic cell count and drug residues evaluation in organic bovine milk.] Microrganismos patogênicos, celularidade e resíduos de antimicrobianos no leite produzido no sistema orgânico. Pesquisa Veterinária Brasileira 29(1):52-58. Disciplina de Enfermidades Infecciosas dos Animais, Departamento de Higiene Veterinária e Saúde Pública, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista, Botucatu, SP 18618-000, Brazil. E-mail: mgribeiro@fmvz.unesp.br In last years increase the importance of milk quality and conditions of bovine milking. Simultaneously, increase the interest about organic milk and derivates. The aim of present study was investigate the milk pathogens, sensitivity and multiple drug resistance of isolates, somatic cell count and residues of drugs in milk, from cattle with and without mastitis, come from four little organic dairy farms in State of São Paulo, Brazil. Were used 148 cattle on the middle period of lactation. From these, two showed clinical mastitis, 72 subclinical mastitis and 74 without signs of mammary inflammation (control). Staphylococcus aureus (25.7%), Streptococcus spp. (21.4%), Corynebacterium bovis (12.9%), Streptococcus agalactiae (4.3%) and Staphylococcus spp (4,3%) were the more-frequent microorganisms isolated from animals with mastitis. Aspergillus spp. was isolated from one animal. Ceftiofur (95.2%), oxacillin (84.2%), gentamicin (76.3%) and cefoperazone (70.3%) were the more effective drugs. High resistance of isolates were found to penicillin (53.5%), ampicillin (41.6%) and neomycin (38.6%). Multiple drug resistance to three or more drug was observed in 40 (39.6%) isolates. Media of somatic cell count encountered in animals with mastitis and controls were 175,742.67cs/mL and 58,227.6 cs/mL, respectively. Antimicrobials residues in milk were detected in four (2.7%) animals. The present findings showed the low somatic cell count of animals, indicative of good quality of milk. However, pointed the need of control measures for contagious pathogens of bovine mastitis and more attention for prohibition of antimicrobial use in organic dairy farms.

• Organic milk mastitis bovine somatic cell count antimicrobials multiresistance

Abstract in Portuguese:

ABSTRACT.- Ribeiro M.G., Geraldo J.S., Langoni H., Lara G.H.B., Siqueira A.K., Salerno T. & Fernandes M.C. 2008. [Pathogenic microorganisms, somatic cell count and drug residues evaluation in organic bovine milk.] Microrganismos patogênicos, celularidade e resíduos de antimicrobianos no leite produzido no sistema orgânico. Pesquisa Veterinária Brasileira 29(1):52-58. Disciplina de Enfermidades Infecciosas dos Animais, Departamento de Higiene Veterinária e Saúde Pública, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista, Botucatu, SP 18618-000, Brazil. E-mail: mgribeiro@fmvz.unesp.br In last years increase the importance of milk quality and conditions of bovine milking. Simultaneously, increase the interest about organic milk and derivates. The aim of present study was investigate the milk pathogens, sensitivity and multiple drug resistance of isolates, somatic cell count and residues of drugs in milk, from cattle with and without mastitis, come from four little organic dairy farms in State of São Paulo, Brazil. Were used 148 cattle on the middle period of lactation. From these, two showed clinical mastitis, 72 subclinical mastitis and 74 without signs of mammary inflammation (control). Staphylococcus aureus (25.7%), Streptococcus spp. (21.4%), Corynebacterium bovis (12.9%), Streptococcus agalactiae (4.3%) and Staphylococcus spp (4,3%) were the more-frequent microorganisms isolated from animals with mastitis. Aspergillus spp. was isolated from one animal. Ceftiofur (95.2%), oxacillin (84.2%), gentamicin (76.3%) and cefoperazone (70.3%) were the more effective drugs. High resistance of isolates were found to penicillin (53.5%), ampicillin (41.6%) and neomycin (38.6%). Multiple drug resistance to three or more drug was observed in 40 (39.6%) isolates. Media of somatic cell count encountered in animals with mastitis and controls were 175,742.67cs/mL and 58,227.6 cs/mL, respectively. Antimicrobials residues in milk were detected in four (2.7%) animals. The present findings showed the low somatic cell count of animals, indicative of good quality of milk. However, pointed the need of control measures for contagious pathogens of bovine mastitis and more attention for prohibition of antimicrobial use in organic dairy farms.